Kuinka säätää laser intensiteetti laser laser mikroskooppi
Siellä on kaksi säätö kertoimet laser intensiteetti % 3a teho ohjaus nuppi akustinen optinen viritettävä suodatin (AOTF).
1. teho ohjaus nuppi % 3a yleensä asetettu arvoon 30 prosenttiin suuri arvo lähtö % laserin käyttöiän laser tulee lyhennetty, ja laser kohina myös syntyvän. AOTF% 2c PMT % 2c etc. pitäisi olla säädetty ensimmäinen to saada hyvä intensiteetti.
2. Acousto-optic viritettävä suodatin: ohjaus viritys valo säde ja intensiteetti, ja can turn on or off laser at millisekunti nopeus tai säädä it to change between 7b{2}}} percent . In this way, different laser beams can be used for each image point, and the required intensity can be selected.
Working principle: The Bragg diffraction effect of sound waves propagating in various media is utilized. When the RF signal with a certain frequency is input, AOTF diffracts the incident polychromatic light, and selects monochromatic light with an incoming wavelength. The wavelength of monochromatic light has a one-to-one correspondence with the RF frequency f, and the wavelength of the output can be changed quickly and randomly by tuning the electrical signal. AOTF can continuously adjust the laser intensity, and has the function of fast adjustment, realizing local stimulation and scanning, and fast wavelength switching (microsecond level). In laser confocal microscope, photomultiplier tube can not increase the laser intensity, but can adjust the photoelectric intensity after imaging, so the brightness of the image can be adjusted by adjusting the voltage of photomultiplier tube. In addition, the size of the pinhole affects the photon quantity and can also adjust the brightness of the image. The photomultiplier tube is a special vacuum tube which is extremely sensitive to ultraviolet light, visible light and near infrared light. It can increase the incoming weak optical signal to 108 times and make the optical signal be measured.
